I. Introduction
- Author: Jerry Workman
- Language: ingliz tilida
- Writing: Lotin yozuvida
- Publisher: toshkent
- Year: 2005
- Views: 240
he first assumption in spectroscopic measurements is that Beer's law
relationship applies between a change in spectrometer response and the
concentration of analyte material present in a sample specimen. The
Bouguer, Lambert, and Beer relationship assumes that the transmission of a
sample within an incident beam is equivalent to 10 exponent the negative
product of the molar extinction coefficient (in L. mol -~ cm-~), multiplied
by the concentration of a molecule in solution (in mol- L-~) times the pathlength (in cm) of the sample in solution. There are some obvious (and not so
obvious) problems with this assumption. The main difficulty in the assumed
relationship is that the molecules often interact, and the extinction coefficient (absorptivity) may vary due to changes in the molecular configuration
of the sample. The obvious temperature, pressure, and interference issues
also create a less than ideal situation for the analyst. However, for many (